Recent Blogs

Introduction: The Impact of pH on Retention, Selectivity, and Peak Shape When developing an HPLC method, one of the most critical factors influencing separation is pH. Whether you’re analyzing pharmaceuticals, biomolecules, or food components, pH affects analyte ionization, retention time, selectivity, and peak shape. Poor pH control can lead to

Introduction: Importance of Gradient Optimization for Difficult Separations When analyzing complex samples, a simple isocratic HPLC method often isn’t enough. If your peaks are co-eluting, broadening, or missing altogether, switching to gradient elution can dramatically improve separation and resolution. However, poorly optimized gradient methods can lead to inconsistent retention times,

Introduction: Why Resolution is Critical for Accurate Results If you’ve ever struggled with overlapping peaks in your HPLC chromatograms, you’re not alone. Resolution (Rs) is one of the most critical parameters in High-Performance Liquid Chromatography (HPLC) because it determines how well two analytes are separated. Poor resolution can lead to

HPLC continues to be an indispensable tool in food analysis, ensuring products are safe, properly labeled, and compliant with global regulations. With advancements in UHPLC, HPLC-MS/MS, and automation, the future of food testing is faster, more efficient, and highly precise.

🔹 For detecting contaminants: HPLC ensures food safety.
🔹 For nutritional labeling: HPLC verifies composition.
🔹 For regulatory compliance: HPLC meets international standards.

Introduction: Importance of Pump Maintenance for System Longevity Your HPLC pump is the heart of your chromatography system. It delivers the mobile phase at a precise and stable flow rate, ensuring accurate retention times, reproducible peak areas, and overall system performance. However, if not properly maintained, your pump can become

Struggling with bad HPLC peaks? Here’s a quick reference guide:

🔹 Peak Tailing → Fix secondary interactions, reduce injection volume, and optimize tubing connections.
🔹 Broad Peaks → Check column health, optimize mobile phase, and improve injection precision.
🔹 Ghost Peaks → Use clean solvents, proper washing protocols, and contaminant-free vials.